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R&D Systems primary antibodies against cd47

Figure 1. <t>CD47</t> levels in human lung adenocarcinoma cells were increased following tumor metastasis (A and B) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the lymph nodes. (C and D) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the liver. (A) and (C) showed representative IHC images for CD47 staining from 14 to 5 lung adenocarcinoma tissue samples, respectively. Data were presented as means ± SDs. **p < 0.01. Scale bar, 50 mm.

Primary Antibodies Against Cd47, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primary+antibodies+against+cd47/Human+CD47+Antibody/pm35663227-168-0-5
Average 93 stars, based on 32 article reviews

primary antibodies against cd47 - by Bioz Stars, 2026-09

93/100 stars

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1) Product Images from "Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis."

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

Journal: Molecular therapy oncolytics

doi: 10.1016/j.omto.2022.04.011

Figure 1. CD47 levels in human lung adenocarcinoma cells were increased following tumor metastasis (A and B) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the lymph nodes. (C and D) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the liver. (A) and (C) showed representative IHC images for CD47 staining from 14 to 5 lung adenocarcinoma tissue samples, respectively. Data were presented as means ± SDs. **p < 0.01. Scale bar, 50 mm.

Figure Legend Snippet: Figure 1. CD47 levels in human lung adenocarcinoma cells were increased following tumor metastasis (A and B) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the lymph nodes. (C and D) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the liver. (A) and (C) showed representative IHC images for CD47 staining from 14 to 5 lung adenocarcinoma tissue samples, respectively. Data were presented as means ± SDs. **p < 0.01. Scale bar, 50 mm.

Techniques Used: Comparison, Expressing, Staining

Figure 2. Induction of CD47 by IFN-g in human lung cancer cells (A) Flow cytometry analysis of CD47 surface expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Right: Representative image; left: quantitative analysis. (B) Immunoﬂuorescence analysis of CD47 expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Scale bar, 20 mm. (C) Western blot analysis of CD47 expression in human lung cancer cell lines after IFN-g treatment. (D) qRT-PCR analysis of CD47 mRNA in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Left: Representative image; right: quantitative analysis. Data from more than 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Figure Legend Snippet: Figure 2. Induction of CD47 by IFN-g in human lung cancer cells (A) Flow cytometry analysis of CD47 surface expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Right: Representative image; left: quantitative analysis. (B) Immunoﬂuorescence analysis of CD47 expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Scale bar, 20 mm. (C) Western blot analysis of CD47 expression in human lung cancer cell lines after IFN-g treatment. (D) qRT-PCR analysis of CD47 mRNA in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Left: Representative image; right: quantitative analysis. Data from more than 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Techniques Used: Flow Cytometry, Expressing, Incubation, Recombinant, Western Blot, Quantitative RT-PCR

Figure 3. Identiﬁcation of genes involved in the IFN signaling pathway that upregulates CD47 expression (A) qRT-PCR analysis of various genes involved in the IFN signaling pathway in A549 cells upon incubation with re- combinant IFN-g (100 ng/mL, 24 h). (B) Normalized luciferase reporter expression of A549 cells transduced with different sets of lentiviral shRNA constructs. Upper: Schematic representation of the CD47-Prom-Fireﬂy Lucif- erase-EF1AProm-Renilla luciferase constructs used to generate the reporter cell lines. Lower: Quantitative anal- ysis. (C) IRF1 protein levels in human lung cancer cells upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Upper: Representative image; lower: quantitative anal- ysis. Data from more than 3 independent experiments were presented as means ± SDs. ***p < 0.001.

Figure Legend Snippet: Figure 3. Identiﬁcation of genes involved in the IFN signaling pathway that upregulates CD47 expression (A) qRT-PCR analysis of various genes involved in the IFN signaling pathway in A549 cells upon incubation with re- combinant IFN-g (100 ng/mL, 24 h). (B) Normalized luciferase reporter expression of A549 cells transduced with different sets of lentiviral shRNA constructs. Upper: Schematic representation of the CD47-Prom-Fireﬂy Lucif- erase-EF1AProm-Renilla luciferase constructs used to generate the reporter cell lines. Lower: Quantitative anal- ysis. (C) IRF1 protein levels in human lung cancer cells upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Upper: Representative image; lower: quantitative anal- ysis. Data from more than 3 independent experiments were presented as means ± SDs. ***p < 0.001.

Techniques Used: Expressing, Quantitative RT-PCR, Incubation, Luciferase, Transduction, shRNA, Construct, Recombinant

Figure 4. IFN-g induces CD47 expression in lung cancer cells through IRF-1 (A) Sequence of the CD47 promoter showing the position of the putative IRF-1-binding site. (B) Reporter assay for putative IRF-1 binding. The results were normalized to relative luciferase units (RLUs). (C) ChIP assay analyzing the CD47 promoter in A549 cells. The results were normalized to the input. (D and E) IRF-1 mRNA (D) and protein (E) levels in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment. (F and G) CD47 level in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment detected by ﬂow cytometry (F) and immunoﬂuorescence (G). Scale bar, 10 mm. (H) Western blot analysis of CD47 levels in A549 cells transfected with IRF-1-speciﬁc or scramble shRNA. Left: Representative image; right: quantitative analysis. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Figure Legend Snippet: Figure 4. IFN-g induces CD47 expression in lung cancer cells through IRF-1 (A) Sequence of the CD47 promoter showing the position of the putative IRF-1-binding site. (B) Reporter assay for putative IRF-1 binding. The results were normalized to relative luciferase units (RLUs). (C) ChIP assay analyzing the CD47 promoter in A549 cells. The results were normalized to the input. (D and E) IRF-1 mRNA (D) and protein (E) levels in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment. (F and G) CD47 level in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment detected by ﬂow cytometry (F) and immunoﬂuorescence (G). Scale bar, 10 mm. (H) Western blot analysis of CD47 levels in A549 cells transfected with IRF-1-speciﬁc or scramble shRNA. Left: Representative image; right: quantitative analysis. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Techniques Used: Expressing, Sequencing, Binding Assay, Reporter Assay, Luciferase, Transfection, shRNA, Cytometry, Western Blot

Figure 5. IFN-g promotes lung cancer cell metastasis by upregulating CD47 expression in vitro (A) Scratch-wound healing assay in A549 cells (A549-WT) and CD47-knockout A549 cells (A549-CD47-KO) were treated with/without IFN-g. (B) Transwell assay in A549 cells (A549-WT) and A549-CD47-KO cells were treated with/without IFN-g. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar, 200 mm.

Figure Legend Snippet: Figure 5. IFN-g promotes lung cancer cell metastasis by upregulating CD47 expression in vitro (A) Scratch-wound healing assay in A549 cells (A549-WT) and CD47-knockout A549 cells (A549-CD47-KO) were treated with/without IFN-g. (B) Transwell assay in A549 cells (A549-WT) and A549-CD47-KO cells were treated with/without IFN-g. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar, 200 mm.

Techniques Used: Expressing, In Vitro, Wound Healing Assay, Knock-Out, Transwell Assay

Figure 6. IFN-g promotes human lung cancer cell metastasis in immunodeﬁcient mice by upregulating CD47 (A) A549 cells (A549-WT) and A549-CD47-KO were engrafted in the lungs of the BALB/c-nude mice. Three weeks post-engraftment, the mice were randomly divided into 2 groups. One group was administered with IFN-g (10 ng/mouse, injected once every 2 days) (A549-WT + IFN-g; A549-CD47-KO + IFN-g), and the other group without IFN-g injection was served as control (A549-WT; A549-CD47-KO). After 5 weeks, the mice were sacriﬁced to analyze tumor growth and metastasis. (B and C) H&E staining and human CD47 immune staining in mouse lungs (B) and livers (C). In both (B) and (C), left: representative images; right: quantiﬁcation of tumor nodules. Data were presented as means ± SDs. *p < 0.05, ***p < 0.001. NS, no signiﬁcance.

Figure Legend Snippet: Figure 6. IFN-g promotes human lung cancer cell metastasis in immunodeﬁcient mice by upregulating CD47 (A) A549 cells (A549-WT) and A549-CD47-KO were engrafted in the lungs of the BALB/c-nude mice. Three weeks post-engraftment, the mice were randomly divided into 2 groups. One group was administered with IFN-g (10 ng/mouse, injected once every 2 days) (A549-WT + IFN-g; A549-CD47-KO + IFN-g), and the other group without IFN-g injection was served as control (A549-WT; A549-CD47-KO). After 5 weeks, the mice were sacriﬁced to analyze tumor growth and metastasis. (B and C) H&E staining and human CD47 immune staining in mouse lungs (B) and livers (C). In both (B) and (C), left: representative images; right: quantiﬁcation of tumor nodules. Data were presented as means ± SDs. *p < 0.05, ***p < 0.001. NS, no signiﬁcance.

Techniques Used: Injection, Control, Staining

Comparison:

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis
Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Expressing:

Staining:

Flow Cytometry:

Incubation:

Recombinant:

Western Blot:

Quantitative RT-PCR:

Luciferase:

Transduction:

shRNA:

Construct:

Sequencing:

Binding Assay:

Reporter Assay:

Transfection:

Cytometry:

In Vitro:

Wound Healing Assay:

Knock-Out:

Transwell Assay:

Injection:

Control:

Immunofluorescence:

Image Search Results

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 1. CD47 levels in human lung adenocarcinoma cells were increased following tumor metastasis (A and B) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the lymph nodes. (C and D) Comparison of CD47 expression between primary lung adenocarcinoma and lung adenocarcinoma metastases in the liver. (A) and (C) showed representative IHC images for CD47 staining from 14 to 5 lung adenocarcinoma tissue samples, respectively. Data were presented as means ± SDs. **p < 0.01. Scale bar, 50 mm.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Comparison, Expressing, Staining

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 2. Induction of CD47 by IFN-g in human lung cancer cells (A) Flow cytometry analysis of CD47 surface expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Right: Representative image; left: quantitative analysis. (B) Immunoﬂuorescence analysis of CD47 expression in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Scale bar, 20 mm. (C) Western blot analysis of CD47 expression in human lung cancer cell lines after IFN-g treatment. (D) qRT-PCR analysis of CD47 mRNA in human lung cancer cell lines upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Left: Representative image; right: quantitative analysis. Data from more than 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Flow Cytometry, Expressing, Incubation, Recombinant, Western Blot, Quantitative RT-PCR

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 3. Identiﬁcation of genes involved in the IFN signaling pathway that upregulates CD47 expression (A) qRT-PCR analysis of various genes involved in the IFN signaling pathway in A549 cells upon incubation with re- combinant IFN-g (100 ng/mL, 24 h). (B) Normalized luciferase reporter expression of A549 cells transduced with different sets of lentiviral shRNA constructs. Upper: Schematic representation of the CD47-Prom-Fireﬂy Lucif- erase-EF1AProm-Renilla luciferase constructs used to generate the reporter cell lines. Lower: Quantitative anal- ysis. (C) IRF1 protein levels in human lung cancer cells upon incubation with recombinant IFN-g (100 ng/mL, 24 h). Upper: Representative image; lower: quantitative anal- ysis. Data from more than 3 independent experiments were presented as means ± SDs. ***p < 0.001.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Expressing, Quantitative RT-PCR, Incubation, Luciferase, Transduction, shRNA, Construct, Recombinant

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 4. IFN-g induces CD47 expression in lung cancer cells through IRF-1 (A) Sequence of the CD47 promoter showing the position of the putative IRF-1-binding site. (B) Reporter assay for putative IRF-1 binding. The results were normalized to relative luciferase units (RLUs). (C) ChIP assay analyzing the CD47 promoter in A549 cells. The results were normalized to the input. (D and E) IRF-1 mRNA (D) and protein (E) levels in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment. (F and G) CD47 level in A549 cells transfected with IRF-1-speciﬁc shRNA or scramble shRNA after IFN-g treatment detected by ﬂow cytometry (F) and immunoﬂuorescence (G). Scale bar, 10 mm. (H) Western blot analysis of CD47 levels in A549 cells transfected with IRF-1-speciﬁc or scramble shRNA. Left: Representative image; right: quantitative analysis. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Expressing, Sequencing, Binding Assay, Reporter Assay, Luciferase, Transfection, shRNA, Cytometry, Western Blot

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 5. IFN-g promotes lung cancer cell metastasis by upregulating CD47 expression in vitro (A) Scratch-wound healing assay in A549 cells (A549-WT) and CD47-knockout A549 cells (A549-CD47-KO) were treated with/without IFN-g. (B) Transwell assay in A549 cells (A549-WT) and A549-CD47-KO cells were treated with/without IFN-g. Data from 3 independent experiments were presented as means ± SDs. **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale bar, 200 mm.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Expressing, In Vitro, Wound Healing Assay, Knock-Out, Transwell Assay

Journal: Molecular therapy oncolytics

Article Title: Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis.

doi: 10.1016/j.omto.2022.04.011

Figure Lengend Snippet: Figure 6. IFN-g promotes human lung cancer cell metastasis in immunodeﬁcient mice by upregulating CD47 (A) A549 cells (A549-WT) and A549-CD47-KO were engrafted in the lungs of the BALB/c-nude mice. Three weeks post-engraftment, the mice were randomly divided into 2 groups. One group was administered with IFN-g (10 ng/mouse, injected once every 2 days) (A549-WT + IFN-g; A549-CD47-KO + IFN-g), and the other group without IFN-g injection was served as control (A549-WT; A549-CD47-KO). After 5 weeks, the mice were sacriﬁced to analyze tumor growth and metastasis. (B and C) H&E staining and human CD47 immune staining in mouse lungs (B) and livers (C). In both (B) and (C), left: representative images; right: quantiﬁcation of tumor nodules. Data were presented as means ± SDs. *p < 0.05, ***p < 0.001. NS, no signiﬁcance.

Article Snippet: Primary antibodies against CD47 (AF4670, R&D Systems, Minneapolis, MN, USA), PD-L1 (#13684, Cell Signaling Technology) and IRF1 (11335-1-AP, Proteintech) were used.

Techniques: Injection, Control, Staining

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: Primers and siRNA sequences.

Article Snippet: Subsequently the cells were blocked with 5% BSA for 1 h. The cell samples were incubated with the primary antibody against CD47 (Santa Cruz Biotechnology (SCBT), Dallas, USA, #sc-12730) at 4 °C overnight.

Techniques: Negative Control

IFN-γ up-regulated CD47 expression. In A549 and NCI-H1975 cells, RT-PCR was to detect mRNA expression of CD47 (a) , western blot was applied to detect the CD47 protein expression (b) , flow cytometry was used to detect cell surface expression of CD47 (c) . ( d) After treatment with IFN-γ (10 ng/ml) for 3, 6, 12, 24 h in two cancer cell lines, the surface protein expression of CD47 was detected by flow cytometry, respectively. *, P < 0.05; **, P < 0.01; ns, nonsignificant.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: IFN-γ up-regulated CD47 expression. In A549 and NCI-H1975 cells, RT-PCR was to detect mRNA expression of CD47 (a) , western blot was applied to detect the CD47 protein expression (b) , flow cytometry was used to detect cell surface expression of CD47 (c) . ( d) After treatment with IFN-γ (10 ng/ml) for 3, 6, 12, 24 h in two cancer cell lines, the surface protein expression of CD47 was detected by flow cytometry, respectively. *, P < 0.05; **, P < 0.01; ns, nonsignificant.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry

Ruxolitinib inhibited CD47 up-regulation induced by IFN-γ. A549 and NCI-H1975 cells were pretreated with ruxolitinib (1 μM) for 1 hours followed by treatment with IFN-γ (10 ng/ml) for 24 h. The mRNA level of CD47 was detected by RT-PCR (a) , Immunofluorescence was used to detected CD47 expression in different treatment groups. The Hoechst 33342 was to stain the cell nuclei and anti-CD47 antibody was to stain CD47 protein. Scale bar: 250 μm ( b), Western blot analysis showed p-STAT1 expression and CD47 expression ( c) , The surface protein expression of CD47 was determined by flow cytometry (d). ( e) After A549 cells were treated with or without ruxolitinib, co-culture experiment was conducted by flow cytometry and then the relatively phagocytosis index was calculated. *, P < 0.05; **, P < 0.01.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: Ruxolitinib inhibited CD47 up-regulation induced by IFN-γ. A549 and NCI-H1975 cells were pretreated with ruxolitinib (1 μM) for 1 hours followed by treatment with IFN-γ (10 ng/ml) for 24 h. The mRNA level of CD47 was detected by RT-PCR (a) , Immunofluorescence was used to detected CD47 expression in different treatment groups. The Hoechst 33342 was to stain the cell nuclei and anti-CD47 antibody was to stain CD47 protein. Scale bar: 250 μm ( b), Western blot analysis showed p-STAT1 expression and CD47 expression ( c) , The surface protein expression of CD47 was determined by flow cytometry (d). ( e) After A549 cells were treated with or without ruxolitinib, co-culture experiment was conducted by flow cytometry and then the relatively phagocytosis index was calculated. *, P < 0.05; **, P < 0.01.

Techniques: Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Expressing, Staining, Western Blot, Flow Cytometry, Co-Culture Assay

Knockdown of JAK1 significantly inhibits CD47 up-regulation induced by IFN-γ. (a-b) After successfully knockdown of JAK1 or JAK2, the cell surface CD47 expression with or without IFN-γ exposure was detected by flow cytometry in A549 and NCI-H1975 cells. ( c) The JAK1/2 and CD47 expression was showed by western blot after knockdown of JAK1/2 with or without IFN-γ treatment. ( d) SIRPα binding assay was conducted to exhibit the binding ability of CD47 to SIRPα in A549 cells after silenced JAK1. ( e) In A549 cells and BMDMs co-culture system, the phagocytosis index changes after knockdown JAK1 combined with or without IFN-γ were analyzed by flow cytometry. *, P < 0.05; **, P < 0.01.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: Knockdown of JAK1 significantly inhibits CD47 up-regulation induced by IFN-γ. (a-b) After successfully knockdown of JAK1 or JAK2, the cell surface CD47 expression with or without IFN-γ exposure was detected by flow cytometry in A549 and NCI-H1975 cells. ( c) The JAK1/2 and CD47 expression was showed by western blot after knockdown of JAK1/2 with or without IFN-γ treatment. ( d) SIRPα binding assay was conducted to exhibit the binding ability of CD47 to SIRPα in A549 cells after silenced JAK1. ( e) In A549 cells and BMDMs co-culture system, the phagocytosis index changes after knockdown JAK1 combined with or without IFN-γ were analyzed by flow cytometry. *, P < 0.05; **, P < 0.01.

Techniques: Knockdown, Expressing, Flow Cytometry, Western Blot, Binding Assay, Co-Culture Assay

Knockdown of STAT1 inhibited CD47 up-regulation induced by IFN-γ. (a) The protein expression of STAT1 and CD47 was analyzed by western blot after knockdown of STAT1 by siRNA in A549 and NCI-H1975 cells. ( b) The cell surface CD47 expression was revealed by flow cytometry after knockdown of STAT1 with or without IFN-γ treatment. (c) Flow cytometry was used to detect the surface expression of CD47 after the STAT1 inhibitor fludarabine treatment. (d) After knockdown of STAT1, the binding of CD47 to SIRPα was determined by flow cytometry in A549 cells. (e) The relative phagocytosis index of BMDMs to A549 cells was calculated based on flow cytometry result. *, P < 0.05; **, P < 0.01.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: Knockdown of STAT1 inhibited CD47 up-regulation induced by IFN-γ. (a) The protein expression of STAT1 and CD47 was analyzed by western blot after knockdown of STAT1 by siRNA in A549 and NCI-H1975 cells. ( b) The cell surface CD47 expression was revealed by flow cytometry after knockdown of STAT1 with or without IFN-γ treatment. (c) Flow cytometry was used to detect the surface expression of CD47 after the STAT1 inhibitor fludarabine treatment. (d) After knockdown of STAT1, the binding of CD47 to SIRPα was determined by flow cytometry in A549 cells. (e) The relative phagocytosis index of BMDMs to A549 cells was calculated based on flow cytometry result. *, P < 0.05; **, P < 0.01.

Techniques: Knockdown, Expressing, Western Blot, Flow Cytometry, Binding Assay

Knockdown of IRF1 inhibited CD47 up-regulation induced by IFN-γ. (a) The protein expression of IRF1 and CD47 was analyzed by western blot analysis after knockdown of IRF1 by siRNA in A549 and NCI-H1975 cells. ( b) Surface expression of CD47 was detected by flow cytometry after silenced IRF1 in two lung cancer cell lines. ( c) SIRPα binding assay was presented to showed CD47 binding ability to SIRPα after knockdown of IRF1 in A549 cells. ( d) Co-culture A549 cells with BMDMs, the phagocytosis index change was measured based on flow cytometry result. *, P < 0.05; **, P < 0.01.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: Knockdown of IRF1 inhibited CD47 up-regulation induced by IFN-γ. (a) The protein expression of IRF1 and CD47 was analyzed by western blot analysis after knockdown of IRF1 by siRNA in A549 and NCI-H1975 cells. ( b) Surface expression of CD47 was detected by flow cytometry after silenced IRF1 in two lung cancer cell lines. ( c) SIRPα binding assay was presented to showed CD47 binding ability to SIRPα after knockdown of IRF1 in A549 cells. ( d) Co-culture A549 cells with BMDMs, the phagocytosis index change was measured based on flow cytometry result. *, P < 0.05; **, P < 0.01.

Techniques: Knockdown, Expressing, Western Blot, Flow Cytometry, Binding Assay, Co-Culture Assay

IFN-γ induced CD47 up-regulation was a widespread phenomenon in cancer cells. (a) The correlations between CD47 and IFNG in different cancer cell types were analyzed by calculating the Pearson correlation coefficients. ( b) In different cancer cell lines, the up-regulation of CD47 induced by IFN-γ was detected by flow cytometry. *, P < 0.05; **, P < 0.01.

Journal: Translational Oncology

Article Title: Regulation of CD47 expression by interferon-gamma in cancer cells

doi: 10.1016/j.tranon.2021.101162

Figure Lengend Snippet: IFN-γ induced CD47 up-regulation was a widespread phenomenon in cancer cells. (a) The correlations between CD47 and IFNG in different cancer cell types were analyzed by calculating the Pearson correlation coefficients. ( b) In different cancer cell lines, the up-regulation of CD47 induced by IFN-γ was detected by flow cytometry. *, P < 0.05; **, P < 0.01.

Techniques: Flow Cytometry

Increased macrophage migration to pancreatic islet cells with the reduction of CD47 expression under streptozotocin treatment. A: The experimental design. Mice were treated by five daily intraperitoneal injections of streptozotocin (STZ) to construct a diabetes model; B: Macrophage infiltration into pancreatic islet cells which was indicated by increased F4/80 labeling accompanied by decreased insulin secretion in STZ treated cells; C: Statistical data; D and E: CD47 expression decreased under STZ condition. Student's t -test was performed. a P < 0.01 vs CTL. CD47: Cluster of differentiation 47; STZ: Streptozotocin; CFSE: Carboxy fluorescein succinimidyl ester.

Journal: World Journal of Diabetes

Article Title: CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes

doi: 10.4239/wjd.v11.i6.239

Figure Lengend Snippet: Increased macrophage migration to pancreatic islet cells with the reduction of CD47 expression under streptozotocin treatment. A: The experimental design. Mice were treated by five daily intraperitoneal injections of streptozotocin (STZ) to construct a diabetes model; B: Macrophage infiltration into pancreatic islet cells which was indicated by increased F4/80 labeling accompanied by decreased insulin secretion in STZ treated cells; C: Statistical data; D and E: CD47 expression decreased under STZ condition. Student's t -test was performed. a P < 0.01 vs CTL. CD47: Cluster of differentiation 47; STZ: Streptozotocin; CFSE: Carboxy fluorescein succinimidyl ester.

Article Snippet: Primary antibodies against CD47 and GAPDH were obtained from Santa Cruz BioTechnology (San Diego, CA, United States).

Techniques: Migration, Expressing, Construct, Labeling

Macrophage phagocytosis assay in vitro . A and B: Flow cytometry results displayed declined CD47 expression of Min6 cells. C: Insulin secretion decreased with STZ stimulation. D: More LPS activated macrophages were recruited to phagocyte CD47 down-regulated Min6 cells. Arrows indicate phagocytosis. Student's t -test was performed. a P < 0.01 vs CTL. CD47: Cluster of differentiation 47; STZ: Streptozotocin; CFSE: Carboxy fluorescein succinimidyl ester.

Journal: World Journal of Diabetes

Article Title: CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes

doi: 10.4239/wjd.v11.i6.239

Figure Lengend Snippet: Macrophage phagocytosis assay in vitro . A and B: Flow cytometry results displayed declined CD47 expression of Min6 cells. C: Insulin secretion decreased with STZ stimulation. D: More LPS activated macrophages were recruited to phagocyte CD47 down-regulated Min6 cells. Arrows indicate phagocytosis. Student's t -test was performed. a P < 0.01 vs CTL. CD47: Cluster of differentiation 47; STZ: Streptozotocin; CFSE: Carboxy fluorescein succinimidyl ester.

Article Snippet: Primary antibodies against CD47 and GAPDH were obtained from Santa Cruz BioTechnology (San Diego, CA, United States).

Techniques: Phagocytosis Assay, In Vitro, Flow Cytometry, Expressing

Macrophage phagocytosis is increased by CD47 siRNA transfection while recovered with CD47 overexpression. A and B: Western blot analysis revealing CD47 relative protein expression with CD47 siRNA transfection and statistical data; C: Macrophage phagocytosis was enhanced when Min6 cells were transfected with CD47 siRNA; D and E: Western blot analysis indicating CD47 relative protein expression when transfection with CD47 open reading frame (ORF; CD47 overexpression) and statistical data; F: Macrophage phagocytosis was impaired by CD47 ORF transfection under STZ condition. GAPDH served as a loading control. Western blot analysis represents the results of three independent experiments. Student's t test was performed. a P < 0.01 vs CTL. CTL: Control. CD47: Cluster of differentiation 47.

Journal: World Journal of Diabetes

Article Title: CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes

doi: 10.4239/wjd.v11.i6.239

Figure Lengend Snippet: Macrophage phagocytosis is increased by CD47 siRNA transfection while recovered with CD47 overexpression. A and B: Western blot analysis revealing CD47 relative protein expression with CD47 siRNA transfection and statistical data; C: Macrophage phagocytosis was enhanced when Min6 cells were transfected with CD47 siRNA; D and E: Western blot analysis indicating CD47 relative protein expression when transfection with CD47 open reading frame (ORF; CD47 overexpression) and statistical data; F: Macrophage phagocytosis was impaired by CD47 ORF transfection under STZ condition. GAPDH served as a loading control. Western blot analysis represents the results of three independent experiments. Student's t test was performed. a P < 0.01 vs CTL. CTL: Control. CD47: Cluster of differentiation 47.

Article Snippet: Primary antibodies against CD47 and GAPDH were obtained from Santa Cruz BioTechnology (San Diego, CA, United States).

Techniques: Transfection, Over Expression, Western Blot, Expressing, Control

Hypothetical model of CD47-SIRPα-regulated inhibition phagocytosis in STZ-induced diabetes. Normally, CD47 is universally expressed on pancreatic islet beta cells. CD47-SIRPα interaction effectively governs macrophage phagocytosis toward healthy self-cells by a “not attach-self” default mode. With the stimulation of STZ, macrophages infiltrate into the pancreatic islet and phagocytose cells when CD47-SIRPα interaction could not be maintained under inflammation condition. “Eat me” signal is transferred with declined expression of CD47 on pancreatic islet cells. CD47: Cluster of differentiation 47; SIRPα: Signal regulatory protein α.

Journal: World Journal of Diabetes

Article Title: CD47 decline in pancreatic islet cells promotes macrophage-mediated phagocytosis in type I diabetes

doi: 10.4239/wjd.v11.i6.239

Figure Lengend Snippet: Hypothetical model of CD47-SIRPα-regulated inhibition phagocytosis in STZ-induced diabetes. Normally, CD47 is universally expressed on pancreatic islet beta cells. CD47-SIRPα interaction effectively governs macrophage phagocytosis toward healthy self-cells by a “not attach-self” default mode. With the stimulation of STZ, macrophages infiltrate into the pancreatic islet and phagocytose cells when CD47-SIRPα interaction could not be maintained under inflammation condition. “Eat me” signal is transferred with declined expression of CD47 on pancreatic islet cells. CD47: Cluster of differentiation 47; SIRPα: Signal regulatory protein α.

Article Snippet: Primary antibodies against CD47 and GAPDH were obtained from Santa Cruz BioTechnology (San Diego, CA, United States).

Techniques: Inhibition, Expressing

Expression levels of miR-200a and CD47 in NPC. Notes: the expression of miR-200a was lower (a) and that of CD47 (b) was higher in NPC tissues than in nasopharyngitis samples. (c) Based on Spearman's correlation analysis, miR-200a expression was negatively correlated with CD47 levels. The expression of miR-200a was lower (d) while that of CD47 was higher (e) in NPC cells than in NP69 cells. ∗ P < 0.05 vs. the NP69 group. Abbreviations: CD47: cluster of differentiation 47; NC: negative control.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: Expression levels of miR-200a and CD47 in NPC. Notes: the expression of miR-200a was lower (a) and that of CD47 (b) was higher in NPC tissues than in nasopharyngitis samples. (c) Based on Spearman's correlation analysis, miR-200a expression was negatively correlated with CD47 levels. The expression of miR-200a was lower (d) while that of CD47 was higher (e) in NPC cells than in NP69 cells. ∗ P < 0.05 vs. the NP69 group. Abbreviations: CD47: cluster of differentiation 47; NC: negative control.

Article Snippet: After incubation with primary antibodies against CD47 (1 : 100, Santa Cruz, Dallas, TX, USA) at 37°C for 30 min and 4°C overnight, the corresponding secondary antibody (Santa Cruz, Dallas, TX, USA) was added for 30 min at 37°C.

Techniques: Expressing, Negative Control

Expression of CD47 in nasopharyngeal carcinoma and nasopharyngitis tissues.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: Expression of CD47 in nasopharyngeal carcinoma and nasopharyngitis tissues.

Techniques: Expressing, Paraffin-embedded Immunohistochemistry

miR-200a directly regulates CD47 expression in NPC cells. Notes: (a, b) based on a dual-luciferase assay, miR-200a was predicted to directly bind to the 3′UTR of CD47 mRNA, and cotransfection of the miR-200a mimic and wild-type promoter resulted in decreased luciferase activity. (c, d) At the mRNA level, CD47 expression was inhibited by the CD47 siRNA in both cell lines. (e, f) At the protein level, CD47 expression was inhibited by the miR-200a mimic in both cell lines. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: UTR: untranslated regions; WT: wild type; MUT: mutant-type; CD47: cluster of differentiation 47.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: miR-200a directly regulates CD47 expression in NPC cells. Notes: (a, b) based on a dual-luciferase assay, miR-200a was predicted to directly bind to the 3′UTR of CD47 mRNA, and cotransfection of the miR-200a mimic and wild-type promoter resulted in decreased luciferase activity. (c, d) At the mRNA level, CD47 expression was inhibited by the CD47 siRNA in both cell lines. (e, f) At the protein level, CD47 expression was inhibited by the miR-200a mimic in both cell lines. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: UTR: untranslated regions; WT: wild type; MUT: mutant-type; CD47: cluster of differentiation 47.

Techniques: Expressing, Luciferase, Cotransfection, Activity Assay, Mutagenesis

Proliferation and colony formation in CNE1 and CNE2 cells. Notes: (a, b) transfection of miR-200a and CD47 siRNA suppressed proliferation and (c, d) colony formation. Cotransfection with CD47 siRNA and miR-200a inhibitor rescued the effects of CD47 knockdown in CNE1 and CNE2 cells. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: CD47: cluster of differentiation 47; EdU: 5-ethynyl-2′-deoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NC: negative control.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: Proliferation and colony formation in CNE1 and CNE2 cells. Notes: (a, b) transfection of miR-200a and CD47 siRNA suppressed proliferation and (c, d) colony formation. Cotransfection with CD47 siRNA and miR-200a inhibitor rescued the effects of CD47 knockdown in CNE1 and CNE2 cells. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: CD47: cluster of differentiation 47; EdU: 5-ethynyl-2′-deoxyuridine; DAPI: 4′,6-diamidino-2-phenylindole; NC: negative control.

Techniques: Transfection, Cotransfection, Knockdown, Negative Control

Migration and invasion of CNE1 and CNE2 cells. Notes: transfection with miR-200a and CD47 siRNA inhibited migration (a, c) and invasion (b, d) in CNE1 and CNE2 cells. Cotransfection with CD47 siRNA and miR-200a inhibitor rescued the effect of CD47 knockdown on the metastatic potential of CNE1 and CNE2 cells. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: CD47: cluster of differentiation 47; NC: negative control.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: Migration and invasion of CNE1 and CNE2 cells. Notes: transfection with miR-200a and CD47 siRNA inhibited migration (a, c) and invasion (b, d) in CNE1 and CNE2 cells. Cotransfection with CD47 siRNA and miR-200a inhibitor rescued the effect of CD47 knockdown on the metastatic potential of CNE1 and CNE2 cells. ∗ P < 0.05 vs. the miR-NC group. ∗∗ P < 0.05 vs. the miR-200a inhibitor group. Abbreviations: CD47: cluster of differentiation 47; NC: negative control.

Techniques: Migration, Transfection, Cotransfection, Knockdown, Negative Control

Anti-CD47 antibody and miR-200a promote phagocytosis of NPC cells by human macrophages. Notes: (a) human peripheral blood- (PB-) derived macrophages (green) are shown phagocytosing NPC cells (red) in the presence of a blocking anti-CD47 monoclonal antibody (B6H12) or IgG1 isotype control antibody. (b) Phagocytosed NPC cells (red) are shown in the miR-200a mimic and miR-NC groups. ∗ P < 0.01. Abbreviations: CD47: cluster of differentiation 47; PBMC: peripheral blood mononuclear cell; IgG: immunoglobulin G.

Journal: BioMed Research International

Article Title: MicroRNA-200a Promotes Phagocytosis of Macrophages and Suppresses Cell Proliferation, Migration, and Invasion in Nasopharyngeal Carcinoma by Targeting CD47

doi: 10.1155/2020/3723781

Figure Lengend Snippet: Anti-CD47 antibody and miR-200a promote phagocytosis of NPC cells by human macrophages. Notes: (a) human peripheral blood- (PB-) derived macrophages (green) are shown phagocytosing NPC cells (red) in the presence of a blocking anti-CD47 monoclonal antibody (B6H12) or IgG1 isotype control antibody. (b) Phagocytosed NPC cells (red) are shown in the miR-200a mimic and miR-NC groups. ∗ P < 0.01. Abbreviations: CD47: cluster of differentiation 47; PBMC: peripheral blood mononuclear cell; IgG: immunoglobulin G.

Techniques: Derivative Assay, Blocking Assay, Control

Review

primary antibodies against cd47 (R&D Systems)

Structured Review

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1) Product Images from "Human lung adenocarcinoma CD47 is upregulated by interferon-γ and promotes tumor metastasis."

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